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pentr1a addgene plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pentr1a addgene plasmid
    Pentr1a Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pentr1a+addgene+plasmid/pLenti+CMV+Blast+DEST+(706-1)+(Plasmid+%2317451)/pm41843626-273-195-196
    Average 94 stars, based on 107 article reviews
    pentr1a addgene plasmid - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Sequencing:

    Article Title: The ubiquitin ligase CBL and Fas-associated factor 2 cooperate to regulate the innate immune response to M. tuberculosis.
    Article Snippet: 3T3-MCSF Gift from Russell Vance, UC Berkeley Chemicals Middlebrook OADC Thermo Fisher Scientific Cat # b12351 BD DIFCO Middlebrook 7H9 Becton Dickinson (BD) Cat # 271310 BD DIFCO Middlebrook 7H10 Becton Dickinson (BD) Cat # 262710 PTMScan Lys-C Protease Cell Signaling Technology Cat # 39003 Sequencing Grade Modified Trypsin Promega Cat # V5111 Bortezomib Cell Signaling Technology Cat # 2204S cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma Aldrich Cat # 11836170001 Bovine growth serum HyClone SH30541.03 Fetal bovine serum Corning 5-015-CV Phorbol 12-myristate 13-acetate (PMA) Sigma Aldrich Cat # P8139 1α,25-Dihydroxyvitamin D3 (Vitamin D) Santa Cruz Cat # 32222-06-3 SYBR Gold Nucleic Acid Gel Stain Invitrogen Cat # S11494 Poly(ethylene glycol) 8000 Sigma Cat # 81268 Lipofectamine 2000 Invitrogen Cat # 11668030 Rhodamine B Sigma 83689-1G Triton X-100 Sigma T9284-100ML Maxima H Minus Reverse Transcriptase Thermo Scientific Cat # EP0753 CellTrace Far Red Thermo Scientific Cat # C34572 CytoFix Red Lysosomal Stain AAT Bioquest Cat # 76485–168 Consumables 500mg Sep-Pak C18 columns Waters Cat # WAT036790 PureLink RNA Mini Kit Invitrogen Cat # 12183018A 0.45 μm Acrodisc Syringe Filters Pall Cat # 4614 Triton X-100 Sigma T9284-100ML Recombinant DNA pLenti Guide Puro Addgene Plasmid #52963 pENTR1a Addgene Plasmid #17451 pLenti CMV blast DEST Addgene Plasmid #10462 pLenti CMV no selection marker This paper pLKO.1 puro pLenti CMV no selection marker This paper pLKO.1 puro Addgene Plasmid #8453 Software and websites Prism v9.0 GraphPad Rstudio Posit https://posit.co/download/ rstudio-desktop/ Synthego guide design tool Synthego https://design.synthego. com TIDE analysis https://tide.nki.nl/ Other PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013974 March 17, 2026 17 / 27

    Modification:

    Article Title: The ubiquitin ligase CBL and Fas-associated factor 2 cooperate to regulate the innate immune response to M. tuberculosis.
    Article Snippet: 3T3-MCSF Gift from Russell Vance, UC Berkeley Chemicals Middlebrook OADC Thermo Fisher Scientific Cat # b12351 BD DIFCO Middlebrook 7H9 Becton Dickinson (BD) Cat # 271310 BD DIFCO Middlebrook 7H10 Becton Dickinson (BD) Cat # 262710 PTMScan Lys-C Protease Cell Signaling Technology Cat # 39003 Sequencing Grade Modified Trypsin Promega Cat # V5111 Bortezomib Cell Signaling Technology Cat # 2204S cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma Aldrich Cat # 11836170001 Bovine growth serum HyClone SH30541.03 Fetal bovine serum Corning 5-015-CV Phorbol 12-myristate 13-acetate (PMA) Sigma Aldrich Cat # P8139 1α,25-Dihydroxyvitamin D3 (Vitamin D) Santa Cruz Cat # 32222-06-3 SYBR Gold Nucleic Acid Gel Stain Invitrogen Cat # S11494 Poly(ethylene glycol) 8000 Sigma Cat # 81268 Lipofectamine 2000 Invitrogen Cat # 11668030 Rhodamine B Sigma 83689-1G Triton X-100 Sigma T9284-100ML Maxima H Minus Reverse Transcriptase Thermo Scientific Cat # EP0753 CellTrace Far Red Thermo Scientific Cat # C34572 CytoFix Red Lysosomal Stain AAT Bioquest Cat # 76485–168 Consumables 500mg Sep-Pak C18 columns Waters Cat # WAT036790 PureLink RNA Mini Kit Invitrogen Cat # 12183018A 0.45 μm Acrodisc Syringe Filters Pall Cat # 4614 Triton X-100 Sigma T9284-100ML Recombinant DNA pLenti Guide Puro Addgene Plasmid #52963 pENTR1a Addgene Plasmid #17451 pLenti CMV blast DEST Addgene Plasmid #10462 pLenti CMV no selection marker This paper pLKO.1 puro pLenti CMV no selection marker This paper pLKO.1 puro Addgene Plasmid #8453 Software and websites Prism v9.0 GraphPad Rstudio Posit https://posit.co/download/ rstudio-desktop/ Synthego guide design tool Synthego https://design.synthego. com TIDE analysis https://tide.nki.nl/ Other PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013974 March 17, 2026 17 / 27

    Protease Inhibitor:

    Article Title: The ubiquitin ligase CBL and Fas-associated factor 2 cooperate to regulate the innate immune response to M. tuberculosis.
    Article Snippet: 3T3-MCSF Gift from Russell Vance, UC Berkeley Chemicals Middlebrook OADC Thermo Fisher Scientific Cat # b12351 BD DIFCO Middlebrook 7H9 Becton Dickinson (BD) Cat # 271310 BD DIFCO Middlebrook 7H10 Becton Dickinson (BD) Cat # 262710 PTMScan Lys-C Protease Cell Signaling Technology Cat # 39003 Sequencing Grade Modified Trypsin Promega Cat # V5111 Bortezomib Cell Signaling Technology Cat # 2204S cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma Aldrich Cat # 11836170001 Bovine growth serum HyClone SH30541.03 Fetal bovine serum Corning 5-015-CV Phorbol 12-myristate 13-acetate (PMA) Sigma Aldrich Cat # P8139 1α,25-Dihydroxyvitamin D3 (Vitamin D) Santa Cruz Cat # 32222-06-3 SYBR Gold Nucleic Acid Gel Stain Invitrogen Cat # S11494 Poly(ethylene glycol) 8000 Sigma Cat # 81268 Lipofectamine 2000 Invitrogen Cat # 11668030 Rhodamine B Sigma 83689-1G Triton X-100 Sigma T9284-100ML Maxima H Minus Reverse Transcriptase Thermo Scientific Cat # EP0753 CellTrace Far Red Thermo Scientific Cat # C34572 CytoFix Red Lysosomal Stain AAT Bioquest Cat # 76485–168 Consumables 500mg Sep-Pak C18 columns Waters Cat # WAT036790 PureLink RNA Mini Kit Invitrogen Cat # 12183018A 0.45 μm Acrodisc Syringe Filters Pall Cat # 4614 Triton X-100 Sigma T9284-100ML Recombinant DNA pLenti Guide Puro Addgene Plasmid #52963 pENTR1a Addgene Plasmid #17451 pLenti CMV blast DEST Addgene Plasmid #10462 pLenti CMV no selection marker This paper pLKO.1 puro pLenti CMV no selection marker This paper pLKO.1 puro Addgene Plasmid #8453 Software and websites Prism v9.0 GraphPad Rstudio Posit https://posit.co/download/ rstudio-desktop/ Synthego guide design tool Synthego https://design.synthego. com TIDE analysis https://tide.nki.nl/ Other PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013974 March 17, 2026 17 / 27

    Staining:

    Article Title: The ubiquitin ligase CBL and Fas-associated factor 2 cooperate to regulate the innate immune response to M. tuberculosis.
    Article Snippet: 3T3-MCSF Gift from Russell Vance, UC Berkeley Chemicals Middlebrook OADC Thermo Fisher Scientific Cat # b12351 BD DIFCO Middlebrook 7H9 Becton Dickinson (BD) Cat # 271310 BD DIFCO Middlebrook 7H10 Becton Dickinson (BD) Cat # 262710 PTMScan Lys-C Protease Cell Signaling Technology Cat # 39003 Sequencing Grade Modified Trypsin Promega Cat # V5111 Bortezomib Cell Signaling Technology Cat # 2204S cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma Aldrich Cat # 11836170001 Bovine growth serum HyClone SH30541.03 Fetal bovine serum Corning 5-015-CV Phorbol 12-myristate 13-acetate (PMA) Sigma Aldrich Cat # P8139 1α,25-Dihydroxyvitamin D3 (Vitamin D) Santa Cruz Cat # 32222-06-3 SYBR Gold Nucleic Acid Gel Stain Invitrogen Cat # S11494 Poly(ethylene glycol) 8000 Sigma Cat # 81268 Lipofectamine 2000 Invitrogen Cat # 11668030 Rhodamine B Sigma 83689-1G Triton X-100 Sigma T9284-100ML Maxima H Minus Reverse Transcriptase Thermo Scientific Cat # EP0753 CellTrace Far Red Thermo Scientific Cat # C34572 CytoFix Red Lysosomal Stain AAT Bioquest Cat # 76485–168 Consumables 500mg Sep-Pak C18 columns Waters Cat # WAT036790 PureLink RNA Mini Kit Invitrogen Cat # 12183018A 0.45 μm Acrodisc Syringe Filters Pall Cat # 4614 Triton X-100 Sigma T9284-100ML Recombinant DNA pLenti Guide Puro Addgene Plasmid #52963 pENTR1a Addgene Plasmid #17451 pLenti CMV blast DEST Addgene Plasmid #10462 pLenti CMV no selection marker This paper pLKO.1 puro pLenti CMV no selection marker This paper pLKO.1 puro Addgene Plasmid #8453 Software and websites Prism v9.0 GraphPad Rstudio Posit https://posit.co/download/ rstudio-desktop/ Synthego guide design tool Synthego https://design.synthego. com TIDE analysis https://tide.nki.nl/ Other PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013974 March 17, 2026 17 / 27

    Reverse Transcription:

    Article Title: The ubiquitin ligase CBL and Fas-associated factor 2 cooperate to regulate the innate immune response to M. tuberculosis.
    Article Snippet: 3T3-MCSF Gift from Russell Vance, UC Berkeley Chemicals Middlebrook OADC Thermo Fisher Scientific Cat # b12351 BD DIFCO Middlebrook 7H9 Becton Dickinson (BD) Cat # 271310 BD DIFCO Middlebrook 7H10 Becton Dickinson (BD) Cat # 262710 PTMScan Lys-C Protease Cell Signaling Technology Cat # 39003 Sequencing Grade Modified Trypsin Promega Cat # V5111 Bortezomib Cell Signaling Technology Cat # 2204S cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma Aldrich Cat # 11836170001 Bovine growth serum HyClone SH30541.03 Fetal bovine serum Corning 5-015-CV Phorbol 12-myristate 13-acetate (PMA) Sigma Aldrich Cat # P8139 1α,25-Dihydroxyvitamin D3 (Vitamin D) Santa Cruz Cat # 32222-06-3 SYBR Gold Nucleic Acid Gel Stain Invitrogen Cat # S11494 Poly(ethylene glycol) 8000 Sigma Cat # 81268 Lipofectamine 2000 Invitrogen Cat # 11668030 Rhodamine B Sigma 83689-1G Triton X-100 Sigma T9284-100ML Maxima H Minus Reverse Transcriptase Thermo Scientific Cat # EP0753 CellTrace Far Red Thermo Scientific Cat # C34572 CytoFix Red Lysosomal Stain AAT Bioquest Cat # 76485–168 Consumables 500mg Sep-Pak C18 columns Waters Cat # WAT036790 PureLink RNA Mini Kit Invitrogen Cat # 12183018A 0.45 μm Acrodisc Syringe Filters Pall Cat # 4614 Triton X-100 Sigma T9284-100ML Recombinant DNA pLenti Guide Puro Addgene Plasmid #52963 pENTR1a Addgene Plasmid #17451 pLenti CMV blast DEST Addgene Plasmid #10462 pLenti CMV no selection marker This paper pLKO.1 puro pLenti CMV no selection marker This paper pLKO.1 puro Addgene Plasmid #8453 Software and websites Prism v9.0 GraphPad Rstudio Posit https://posit.co/download/ rstudio-desktop/ Synthego guide design tool Synthego https://design.synthego. com TIDE analysis https://tide.nki.nl/ Other PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013974 March 17, 2026 17 / 27

    Recombinant:

    Article Title: The ubiquitin ligase CBL and Fas-associated factor 2 cooperate to regulate the innate immune response to M. tuberculosis.
    Article Snippet: 3T3-MCSF Gift from Russell Vance, UC Berkeley Chemicals Middlebrook OADC Thermo Fisher Scientific Cat # b12351 BD DIFCO Middlebrook 7H9 Becton Dickinson (BD) Cat # 271310 BD DIFCO Middlebrook 7H10 Becton Dickinson (BD) Cat # 262710 PTMScan Lys-C Protease Cell Signaling Technology Cat # 39003 Sequencing Grade Modified Trypsin Promega Cat # V5111 Bortezomib Cell Signaling Technology Cat # 2204S cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma Aldrich Cat # 11836170001 Bovine growth serum HyClone SH30541.03 Fetal bovine serum Corning 5-015-CV Phorbol 12-myristate 13-acetate (PMA) Sigma Aldrich Cat # P8139 1α,25-Dihydroxyvitamin D3 (Vitamin D) Santa Cruz Cat # 32222-06-3 SYBR Gold Nucleic Acid Gel Stain Invitrogen Cat # S11494 Poly(ethylene glycol) 8000 Sigma Cat # 81268 Lipofectamine 2000 Invitrogen Cat # 11668030 Rhodamine B Sigma 83689-1G Triton X-100 Sigma T9284-100ML Maxima H Minus Reverse Transcriptase Thermo Scientific Cat # EP0753 CellTrace Far Red Thermo Scientific Cat # C34572 CytoFix Red Lysosomal Stain AAT Bioquest Cat # 76485–168 Consumables 500mg Sep-Pak C18 columns Waters Cat # WAT036790 PureLink RNA Mini Kit Invitrogen Cat # 12183018A 0.45 μm Acrodisc Syringe Filters Pall Cat # 4614 Triton X-100 Sigma T9284-100ML Recombinant DNA pLenti Guide Puro Addgene Plasmid #52963 pENTR1a Addgene Plasmid #17451 pLenti CMV blast DEST Addgene Plasmid #10462 pLenti CMV no selection marker This paper pLKO.1 puro pLenti CMV no selection marker This paper pLKO.1 puro Addgene Plasmid #8453 Software and websites Prism v9.0 GraphPad Rstudio Posit https://posit.co/download/ rstudio-desktop/ Synthego guide design tool Synthego https://design.synthego. com TIDE analysis https://tide.nki.nl/ Other PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013974 March 17, 2026 17 / 27

    Plasmid Preparation:

    Article Title: The ubiquitin ligase CBL and Fas-associated factor 2 cooperate to regulate the innate immune response to M. tuberculosis.
    Article Snippet: 3T3-MCSF Gift from Russell Vance, UC Berkeley Chemicals Middlebrook OADC Thermo Fisher Scientific Cat # b12351 BD DIFCO Middlebrook 7H9 Becton Dickinson (BD) Cat # 271310 BD DIFCO Middlebrook 7H10 Becton Dickinson (BD) Cat # 262710 PTMScan Lys-C Protease Cell Signaling Technology Cat # 39003 Sequencing Grade Modified Trypsin Promega Cat # V5111 Bortezomib Cell Signaling Technology Cat # 2204S cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma Aldrich Cat # 11836170001 Bovine growth serum HyClone SH30541.03 Fetal bovine serum Corning 5-015-CV Phorbol 12-myristate 13-acetate (PMA) Sigma Aldrich Cat # P8139 1α,25-Dihydroxyvitamin D3 (Vitamin D) Santa Cruz Cat # 32222-06-3 SYBR Gold Nucleic Acid Gel Stain Invitrogen Cat # S11494 Poly(ethylene glycol) 8000 Sigma Cat # 81268 Lipofectamine 2000 Invitrogen Cat # 11668030 Rhodamine B Sigma 83689-1G Triton X-100 Sigma T9284-100ML Maxima H Minus Reverse Transcriptase Thermo Scientific Cat # EP0753 CellTrace Far Red Thermo Scientific Cat # C34572 CytoFix Red Lysosomal Stain AAT Bioquest Cat # 76485–168 Consumables 500mg Sep-Pak C18 columns Waters Cat # WAT036790 PureLink RNA Mini Kit Invitrogen Cat # 12183018A 0.45 μm Acrodisc Syringe Filters Pall Cat # 4614 Triton X-100 Sigma T9284-100ML Recombinant DNA pLenti Guide Puro Addgene Plasmid #52963 pENTR1a Addgene Plasmid #17451 pLenti CMV blast DEST Addgene Plasmid #10462 pLenti CMV no selection marker This paper pLKO.1 puro pLenti CMV no selection marker This paper pLKO.1 puro Addgene Plasmid #8453 Software and websites Prism v9.0 GraphPad Rstudio Posit https://posit.co/download/ rstudio-desktop/ Synthego guide design tool Synthego https://design.synthego. com TIDE analysis https://tide.nki.nl/ Other PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013974 March 17, 2026 17 / 27

    Selection:

    Article Title: The ubiquitin ligase CBL and Fas-associated factor 2 cooperate to regulate the innate immune response to M. tuberculosis.
    Article Snippet: 3T3-MCSF Gift from Russell Vance, UC Berkeley Chemicals Middlebrook OADC Thermo Fisher Scientific Cat # b12351 BD DIFCO Middlebrook 7H9 Becton Dickinson (BD) Cat # 271310 BD DIFCO Middlebrook 7H10 Becton Dickinson (BD) Cat # 262710 PTMScan Lys-C Protease Cell Signaling Technology Cat # 39003 Sequencing Grade Modified Trypsin Promega Cat # V5111 Bortezomib Cell Signaling Technology Cat # 2204S cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma Aldrich Cat # 11836170001 Bovine growth serum HyClone SH30541.03 Fetal bovine serum Corning 5-015-CV Phorbol 12-myristate 13-acetate (PMA) Sigma Aldrich Cat # P8139 1α,25-Dihydroxyvitamin D3 (Vitamin D) Santa Cruz Cat # 32222-06-3 SYBR Gold Nucleic Acid Gel Stain Invitrogen Cat # S11494 Poly(ethylene glycol) 8000 Sigma Cat # 81268 Lipofectamine 2000 Invitrogen Cat # 11668030 Rhodamine B Sigma 83689-1G Triton X-100 Sigma T9284-100ML Maxima H Minus Reverse Transcriptase Thermo Scientific Cat # EP0753 CellTrace Far Red Thermo Scientific Cat # C34572 CytoFix Red Lysosomal Stain AAT Bioquest Cat # 76485–168 Consumables 500mg Sep-Pak C18 columns Waters Cat # WAT036790 PureLink RNA Mini Kit Invitrogen Cat # 12183018A 0.45 μm Acrodisc Syringe Filters Pall Cat # 4614 Triton X-100 Sigma T9284-100ML Recombinant DNA pLenti Guide Puro Addgene Plasmid #52963 pENTR1a Addgene Plasmid #17451 pLenti CMV blast DEST Addgene Plasmid #10462 pLenti CMV no selection marker This paper pLKO.1 puro pLenti CMV no selection marker This paper pLKO.1 puro Addgene Plasmid #8453 Software and websites Prism v9.0 GraphPad Rstudio Posit https://posit.co/download/ rstudio-desktop/ Synthego guide design tool Synthego https://design.synthego. com TIDE analysis https://tide.nki.nl/ Other PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013974 March 17, 2026 17 / 27

    Marker:

    Article Title: The ubiquitin ligase CBL and Fas-associated factor 2 cooperate to regulate the innate immune response to M. tuberculosis.
    Article Snippet: 3T3-MCSF Gift from Russell Vance, UC Berkeley Chemicals Middlebrook OADC Thermo Fisher Scientific Cat # b12351 BD DIFCO Middlebrook 7H9 Becton Dickinson (BD) Cat # 271310 BD DIFCO Middlebrook 7H10 Becton Dickinson (BD) Cat # 262710 PTMScan Lys-C Protease Cell Signaling Technology Cat # 39003 Sequencing Grade Modified Trypsin Promega Cat # V5111 Bortezomib Cell Signaling Technology Cat # 2204S cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma Aldrich Cat # 11836170001 Bovine growth serum HyClone SH30541.03 Fetal bovine serum Corning 5-015-CV Phorbol 12-myristate 13-acetate (PMA) Sigma Aldrich Cat # P8139 1α,25-Dihydroxyvitamin D3 (Vitamin D) Santa Cruz Cat # 32222-06-3 SYBR Gold Nucleic Acid Gel Stain Invitrogen Cat # S11494 Poly(ethylene glycol) 8000 Sigma Cat # 81268 Lipofectamine 2000 Invitrogen Cat # 11668030 Rhodamine B Sigma 83689-1G Triton X-100 Sigma T9284-100ML Maxima H Minus Reverse Transcriptase Thermo Scientific Cat # EP0753 CellTrace Far Red Thermo Scientific Cat # C34572 CytoFix Red Lysosomal Stain AAT Bioquest Cat # 76485–168 Consumables 500mg Sep-Pak C18 columns Waters Cat # WAT036790 PureLink RNA Mini Kit Invitrogen Cat # 12183018A 0.45 μm Acrodisc Syringe Filters Pall Cat # 4614 Triton X-100 Sigma T9284-100ML Recombinant DNA pLenti Guide Puro Addgene Plasmid #52963 pENTR1a Addgene Plasmid #17451 pLenti CMV blast DEST Addgene Plasmid #10462 pLenti CMV no selection marker This paper pLKO.1 puro pLenti CMV no selection marker This paper pLKO.1 puro Addgene Plasmid #8453 Software and websites Prism v9.0 GraphPad Rstudio Posit https://posit.co/download/ rstudio-desktop/ Synthego guide design tool Synthego https://design.synthego. com TIDE analysis https://tide.nki.nl/ Other PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013974 March 17, 2026 17 / 27

    Software:

    Article Title: The ubiquitin ligase CBL and Fas-associated factor 2 cooperate to regulate the innate immune response to M. tuberculosis.
    Article Snippet: 3T3-MCSF Gift from Russell Vance, UC Berkeley Chemicals Middlebrook OADC Thermo Fisher Scientific Cat # b12351 BD DIFCO Middlebrook 7H9 Becton Dickinson (BD) Cat # 271310 BD DIFCO Middlebrook 7H10 Becton Dickinson (BD) Cat # 262710 PTMScan Lys-C Protease Cell Signaling Technology Cat # 39003 Sequencing Grade Modified Trypsin Promega Cat # V5111 Bortezomib Cell Signaling Technology Cat # 2204S cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma Aldrich Cat # 11836170001 Bovine growth serum HyClone SH30541.03 Fetal bovine serum Corning 5-015-CV Phorbol 12-myristate 13-acetate (PMA) Sigma Aldrich Cat # P8139 1α,25-Dihydroxyvitamin D3 (Vitamin D) Santa Cruz Cat # 32222-06-3 SYBR Gold Nucleic Acid Gel Stain Invitrogen Cat # S11494 Poly(ethylene glycol) 8000 Sigma Cat # 81268 Lipofectamine 2000 Invitrogen Cat # 11668030 Rhodamine B Sigma 83689-1G Triton X-100 Sigma T9284-100ML Maxima H Minus Reverse Transcriptase Thermo Scientific Cat # EP0753 CellTrace Far Red Thermo Scientific Cat # C34572 CytoFix Red Lysosomal Stain AAT Bioquest Cat # 76485–168 Consumables 500mg Sep-Pak C18 columns Waters Cat # WAT036790 PureLink RNA Mini Kit Invitrogen Cat # 12183018A 0.45 μm Acrodisc Syringe Filters Pall Cat # 4614 Triton X-100 Sigma T9284-100ML Recombinant DNA pLenti Guide Puro Addgene Plasmid #52963 pENTR1a Addgene Plasmid #17451 pLenti CMV blast DEST Addgene Plasmid #10462 pLenti CMV no selection marker This paper pLKO.1 puro pLenti CMV no selection marker This paper pLKO.1 puro Addgene Plasmid #8453 Software and websites Prism v9.0 GraphPad Rstudio Posit https://posit.co/download/ rstudio-desktop/ Synthego guide design tool Synthego https://design.synthego. com TIDE analysis https://tide.nki.nl/ Other PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1013974 March 17, 2026 17 / 27



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